Schettgen T1, Alt A1, Dewes P1, Kraus T1.
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jul 15;995-996:93-100. doi: 10.1016/j.jchromb.2015.05.023. Epub 2015 May 21.We have developed and validated a simple and sensitive method for the determination of urinary phenol as well as the urinary metabolites of toluene and ethylbenzene in one analytical run. After enzymatic hydrolysis for the cleavage of conjugates overnight, the analytes are extracted from the matrix with a liquid-liquid extraction procedure using toluene as solvent under acidic conditions. The analytes are then derivatised to volatile ethers using N,O-bis(trimethylsilyl) trifluoroacetamid (BSTFA) for cresols and ethylphenols as well as N-tert-butyldimethylsilyl-N-methyltrifluoroacetamid (MTBSTFA) for the determination of phenol. Read More