Pellegrini D1, Onor M1, Degano I2, Bramanti E3.
Talanta. 2014 Dec;130:280-7. doi: 10.1016/j.talanta.2014.07.015. Epub 2014 Jul 15.We developed a novel and straightforward derivatization method for the determination of lactate by reversed phase high-performance liquid chromatography (RP-HPLC) with fluorescence and UV detection in biological matrices as urine and saliva. The derivatization of lactate was achieved employing 9-chloromethyl anthracene (9-CMA) as fluorescence reagent, which has never been previously used to obtain a lactate derivative. Read More