Theron CW1, Labuschagné M, Gudiminchi R, Albertyn J, Smit MS.
FEMS Yeast Res. 2014 Jun;14(4):556-66. doi: 10.1111/1567-1364.12142. Epub 2014 Mar 5.The feasibility of using a single vector to clone a cytochrome P450 monooxygenase (P450) in different yeasts and then compare whole-cell hydroxylase activity was investigated. A broad-range yeast expression vector using the ylTEFp to drive expression of the cloned gene and the scTEFp to drive the hygromycin resistance marker gene was used to clone the genes encoding two self-sufficient P450s, CYP102A1 and CYP505A1. Read More