Caramelo-Nunes C1, Tomaz CT.
Methods Mol Biol. 2015;1286:47-54. doi: 10.1007/978-1-4939-2447-9_5.Liquid chromatography is the method of choice for the purification of plasmid DNA (pDNA), since it is simple, robust, versatile, and highly reproducible. The most important features of a chromatographic procedure are the use of suitable stationary phases and ligands. As conventional purification protocols are being replaced by more sophisticated and selective procedures, the focus changes toward designing and selecting ligands of high affinity and specificity. Read More