Alegria TG1, Meireles DA1, Cussiol JR1, Hugo M2, Trujillo M2, de Oliveira MA3, Miyamoto S4, Queiroz RF4, Valadares NF5, Garratt RC5, Radi R6, Di Mascio P4, Augusto O4, Netto LE7.
Proc Natl Acad Sci U S A. 2016 Dec 27. pii: 201619659. doi: 10.1073/pnas.1619659114. [Epub ahead of print]Organic hydroperoxide resistance (Ohr) enzymes are unique Cys-based, lipoyl-dependent peroxidases. Here, we investigated the involvement of Ohr in bacterial responses toward distinct hydroperoxides. In silico results indicated that fatty acid (but not cholesterol) hydroperoxides docked well into the active site of Ohr from Xylella fastidiosa and were efficiently reduced by the recombinant enzyme as assessed by a lipoamide-lipoamide dehydrogenase-coupled assay. Read More