Peng Y1, Turner NW2, Britt DW3.
Colloids Surf B Biointerfaces. 2014 Jul 1;119:6-13. doi: 10.1016/j.colsurfb.2014.04.015. Epub 2014 Apr 30.Fluoroalcohols such as 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) are strong inducers of protein secondary structure. Here, hydrolyzed (3,3,3-trifluoropropyl)trimethoxysilane (3F) is demonstrated to exhibit greater protein conformation inducing activity than HFIP, which is preserved when 3F is copolymerized in tetraethylorthosilicate (TEOS) sol-gels through protein molecular imprinting. Hydrolyzed 3F formed a pre-polymerization complex with the template protein, β-lactoglobulin, inducing distinct α-helical structures as evidenced by circular dichroism. Read More